Alich TC, Pabst M, Pothmann L, Szalontai B, Faas GC, and Mody I (2021) A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed. PNAS: 118 (6) e2020235118
Abstract: Voltage sensing with genetically expressed optical probes is highly desirable for large-scale recordings of neuronal activity and detection of localized voltage signals in single neurons. Most genetically encodable voltage indicators (GEVI) have drawbacks including slow response, low fluorescence, or excessive bleaching. Here we present a dark quencher GEVI approach (dqGEVI) using a Förster resonance energy transfer pair between a fluorophore glycosylphosphatidylinositol– enhanced green fluorescent protein (GPI-eGFP) on the outer surface of the neuronal membrane and an azo-benzene dye quencher (D3) that rapidly moves in the membrane driven by voltage. In contrast to previous probes, the sensor has a single photon bleaching time constant of ∼40 min, has a high temporal resolution and fidelity for detecting action potential firing at 100 Hz, resolves membrane deand hyperpolarizations of a few millivolts, and has negligible effects on passive membrane properties or synaptic events. The dqGEVI approach should be a valuable tool for optical recordings of subcellular or population membrane potential changes in nerve cells
Find the paper here.
Alich TC, Pabst M, Pothmann L, Szalontai B, Faas GC, and Mody I (2021) A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed. PNAS: 118 (6) e2020235118
Abstract: Voltage sensing with genetically expressed optical probes is highly desirable for large-scale recordings of neuronal activity and detection of localized voltage signals in single neurons. Most genetically encodable voltage indicators (GEVI) have drawbacks including slow response, low fluorescence, or excessive bleaching. Here we present a dark quencher GEVI approach (dqGEVI) using a Förster resonance energy transfer pair between a fluorophore glycosylphosphatidylinositol– enhanced green fluorescent protein (GPI-eGFP) on the outer surface of the neuronal membrane and an azo-benzene dye quencher (D3) that rapidly moves in the membrane driven by voltage. In contrast to previous probes, the sensor has a single photon bleaching time constant of ∼40 min, has a high temporal resolution and fidelity for detecting action potential firing at 100 Hz, resolves membrane deand hyperpolarizations of a few millivolts, and has negligible effects on passive membrane properties or synaptic events. The dqGEVI approach should be a valuable tool for optical recordings of subcellular or population membrane potential changes in nerve cells
Find the paper here.
The BTC offers a rich repertoire of cutting-edge technologies organized into 12 core facilities. Numerous transgenic mouse lines and viral vectors is freely available at our in-house repositories.
The BTC offers a rich repertoire of cutting-edge technologies organized into 12 core facilities. Numerous transgenic mouse lines and viral vectors is freely available at our in-house repositories.
The BTC offers a rich repertoire of cutting-edge technologies organized into 12 core facilities. Numerous transgenic mouse lines and viral vectors is freely available at our in-house repositories.